Phospho erk antibody cst

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August undefined, 2024

WebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 950 publications. Validated for WB, IP, IHC. Highly specific and rigorously validated in-house, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit Monoclonal Antibody (CST #4376) is ready to ship. WebApr 11, 2024 · Dox removal results in loss of Kras G12D expression and ERK activity in iKras PDAC cells. Cells were grown in the presence or absence of Dox for the indicated amounts of time, before being subjected to lysis and immunoblotting (IB) with the indicated antibodies. ... Cell Signaling: 4695S: Anti-Phospho-p44/42 MAPK (Thr202/Tyr204) (p …

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody

WebApr 5, 2024 · Cell lysates were then obtained and submitted to western blot analysis using anti-phospho-ERK (p-ERK) or anti-SMN antibodies. Membranes were stripped and reprobed with anti-ERK (pan-ERK) antibody ... WebCyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a … iphone bookmarks not syncing

Detection and Differentiation of Threonine- and Tyrosine ... - Nature

Webphospho erk antibodies (Cell Signaling Technology Inc) Cell Signaling Technology Inc is a verified supplier Cell Signaling Technology Inc manufactures this product About News … WebImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PERK (phospho T982) antibody (ab192591) Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue labeling PERK (phospho T982) with ab192591 at a 1/50 dilution. The image on the right shows the same antibody preincubated with blocking … WebCell Signaling Technology's Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot. iphone bmw cable

Anti-PERK (phospho T982) antibody (ab192591) Abcam

WebMonoclonal Antibody for studying Thr-P. Cited in 2 publications. Highly specific and rigorously validated in-house, Phospho-Threonine (42H4) Mouse Monoclonal Antibody … WebSpecificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little … iphone bookmarks iconWebApr 12, 2024 · CDK12 primary antibody (CST #11973s) was diluted in reagent dilution 1:1000, and 100 µL of the antibody dilution was added to each well in the microplate. ... Cell lysates were immunoblotted with RNA Pol II antibodies and Ser2 or-Ser5 phospho-specific RNA Pol II antibodies to determine the effective concentration of AU-16770 required to ... iphone bolt

"WebAug 15, 2003 · Activation of AKT and ERK-1/2 was assessed using 1:1000 dilutions of antiphospho-specific antibodies (Cell Signaling Technology). Horseradish peroxidase–conjugated secondary antibodies were used to develop the membrane. The protein bands were detected with Supersignal chemiluminescence substrate (Pierce … " - Phospho erk antibody cst

Phospho erk antibody cst

Phospho-Bad (Ser136) (5D8) Mouse mAb Cell Signaling …

WebThe Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody. This antibody recognizes Human, Mouse, Rat, Bovine, C. elegans/Worm, Drosophila/Arthropod, Hamster, Non-Human Primate, Porcine, Zebrafish/Fish, and Other Mammalian antigen. WebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 1049 publications. Validated for WB, WB, IF, F. Available in 2 sizes. …

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WebProduct overview. Abcam’s ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA kit is designed for the semi-quantitative measurement of ERK1/2 (pT202/Y204) and Total ERK1/2 protein in Human and mouse cells. The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody ... WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase. …

WebAug 3, 2015 · Importantly, the two anti-phospho-ERK1/2 antibodies tested here displayed different selectivities for the presumed monophosphorylated ERK1 and ERK2 species: The monoclonal rabbit antibody... WebPhospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (44-680G) in IHC (P) Immunohistochemistry analysis of ERK1/2 (pTpY185/187) showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left).

WebThis antibody is predicted to react with Xenopus and zebrafish based on 100% sequence homology. 36-8800 is specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. WebMay 31, 2024 · Background We recently reported that STAT1 plays a tumor suppressor role, and ERK was inversely correlation with STAT1 expression in esophageal squamous cell carcinoma (ESCC). Here, we investigated the mechanism(s) that are responsible for the ERK regulates STAT1 in ESCC. Methods We performed the immunoprecipitation (IP) to detect …

WebThis kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels, THUNDER Format: Half-area 96-well or low-volume 384-well plate Formulation: Eu-phospho-ERK (20 uL) Acc-phospho-ERK (80 uL) Eu-Total ERK (5 uL) Acc-Total ERK (20 uL) ERK control lysate (500 uL) 5X Lysis Buffer #1 (5 mL) 10X TR-FRET Detection buffer (250 uL) Gene ...

WebJan 18, 2024 · Phosphorylation of ERK is increased by CCN2. ( A) LECs were stimulated with 100 ng/mL CCN2 for 0, 5, 15 or 30 min, and phosphorylated ERK, total ERK and HSP90 were detected by Western... iphone bookmark 登録WebPhospho-PERK (Thr982) Antibody (PA5-40294) in WB. Oncology reports 2024 - Figure 3. DHA activates the UPRs in PLC cells in vitro . HepG2 and PLC/PRF/5 cells were treated with 40 and 25 uM DHA, respectively, for 1, 6, 12 or 24 h. (A and C) The protein expression levels of UPR-associated molecules were assessed by western blotting. iphone books for seniorsWebPhospho-ERK Assays Extracellular signal-related kinase (ERK1/2 or p42/44) is a kinase in the mitogen-activated protein kinase (MAPK) family and phosphorylation of ERK (p-ERK) can be used as a common end point measurement for the activation of many classes of G protein coupled receptors (GPCR). This chapter addresses t … iphone book apps freeWebThe Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody. This antibody recognizes Human, Mouse, Rat, … iphone bnplWebMay 14, 2024 · After initiating T-cell signaling with the dimerizer, we pulsed the cell conjugates with light after 5 min for 3 min before returning them to the signaling-competent state. ... The resuspended pellet was then incubated with the phospho-ERK1/2 (Thr 202 /Tyr 204) antibody directly conjugated with Alexa Fluor 488 for 45 min at room temperature ... iphone bolt budapestWebPhospho-ERK Assays Extracellular signal-related kinase (ERK1/2 or p42/44) is a kinase in the mitogen-activated protein kinase (MAPK) family and phosphorylation of ERK (p-ERK) … iphone books on tapeWeb(4376) Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb - Cell Signaling Technology - CiteAb. 952 citations – A rabbit monoclonal antibody, raised against Mitogen-activated protein kinase 1 (Human) and Mitogen-activated protein kinase 3 (Human), supplied by Cell Signaling Technology. ... ERK-1, ERK1, ERT2, Extracellular … iphone bold text