Line1 orf2 antibody

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August undefined, 2024

NettetORF1p and ORF2p are LINE1 encoded proteins essential for LINE1 retrotransposition. LINE1s are silenced epigenetically in somatic tissues, and their reactivation has been associated with cancer pathogenesis. Here, we present evidence that nucleolin (NCL) regulates expression of LINE1-ORF1p (L1-ORF1p) in NSCLC cells. NettetAnti-LINE-1 Retrotransposable Element ORF2 Protein [1G4E11] Antibody. This mouse IgG1k monoclonal antibody was generated against bacterially-purified human …

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NettetAnti-LINE1 ORF2 Antibody, clone 1G4E11 Match Criteria: Product Name, Keyword All Photos ( 1) Anti-Nuclease P1 antibody produced in rabbit Match Criteria: Product Name, Keyword All Photos ( 6) NettetAntibodies that detect L1/ORF2 can be used in several scientific applications, including ELISA and Western Blot. These antibodies target L1/ORF2 in Mouse, Human and … tertiary math

Viruses Free Full-Text LINE1-Mediated Reverse Transcription …

NettetGeneral description. LINE-1 retrotransposable element ORF2 protein (UniProt: O00370; also known as EC:2.7.7.49, ORF2p endonuclease, ORF2p) is encoded by the Orf2p … Nettet25. feb. 2024 · Full-length LINE1 encodes two proteins essential for LINE1 retrotransposition: L1ORF1p and L1ORF2p. L1ORF1p is a nucleic acid chaperone with high RNA-binding affinity [ 13 ]. L1ORF2p harbors endonuclease (EN) and reverse-transcriptase activities and a cysteine-rich domain that is also essential for … NettetImmunocytochemistry/ Immunofluorescence: LINE-1 ORF1p Antibody [NBP2-66934] - Staining L1ORF1p in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Product Details Summary Order Details Loading... 100% Guarantee on every product Datasheet Reviews & Publications Protocols & FAQs … trima motor - arbucies - bosch

LINE-1 protein localization and functional dynamics during the …

Line1 orf2 antibody

Genes Free Full-Text Factors Regulating the Activity of LINE1 ...

NettetImmunogen. Synthetic peptide corresponding to Human LINE-1 ORF2p conjugated to keyhole limpet haemocyanin. Two synthetic peptides conjugated to keyhole limpet … NettetAnti-LINE1 ORF2 Antibody, clone 1G4E11 Anti-LINE1 ORF2, clone 1G4E11, Cat. No. MABE1987, is a mouse monoclonal antibody that detects LINE-1 retrotransposable …

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Nettet26. jan. 2016 · LINE-1 (L1) retrotransposons are a source of endogenous reverse transcriptase (RT) activity, which is expressed as part of the L1-encoded ORF2 protein …

NettetORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, … Nettet10. des. 2014 · L1 mobilization relies on the function of two, self-encoded proteins, ORF1 and ORF2. The ORF2 protein contains two characterized domains: endonuclease and reverse transcriptase. Results: Using a bacterially purified endonuclease domain of the human L1 ORF2 protein, we have generated a monoclonal antibody specific to the …

NettetAnti-LINE-1 ORF2p antibody 製品の詳細 Chicken polyclonal to LINE-1 ORF2p 由来種 Chicken 特異性 The two synthetic immunogen peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein. This antibody is intended for use in studying control of L1 retrotransposons. アプリケーション適用あり: ELISA, WB more … Nettet28. aug. 2024 · The kinetics of nascent LINE-1 transcription was consistent with changes at the protein level, as determined by immunofluorescence with an antibody to ORF1p ( Fig. 1d ). ORF1p exhibited the...

NettetAnti-LINE1 ORF2 Antibody, clone 1G4E11. Compare Product No. Clonality Application Species Reactivity Citations SDS; MABE1987: 1G4E11, monoclonal: ELISA, WB, inhibition assay: human: Expand. Hide. Match Criteria: Product Name, Keyword. All Photos (1) Anti-Nuclease P1 antibody produced in rabbit. Compare Product No. Clonality

NettetImmunocytochemistry/ Immunofluorescence: LINE-1 ORF1p Antibody [NBP2-66934] - Staining L1ORF1p in F9 cells (green). Cells were fixed in paraformaldehyde, … tertiary mitigationNettetAnti-Orf2 is directed against mouse ORF2 near a c-terminus position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide … tertiary minerals newsNettetAnti-LINE1 ORF2 Antibody, clone 1G4E11 Anti-LINE1 ORF2, clone 1G4E11, Cat. No. MABE1987, is a mouse monoclonal antibody that detects LINE-1 retrotransposable element ORF2 protein and is tested for use in ELISA, Inhibition Assay, and Western Blotting. - Find MSDS or SDS, a COA, data sheets and more information. trimana culver cityNettetAll lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution Lane 1 : induced bacterially expressed human ORF2 Lane 2 : uninduced bacterially expressed human … trim am reviewsNettet9. jun. 2013 · The L1 expresses a 6-kb bicistronic RNA that encodes the 40 kDa Open Reading Frame-1 RNA-binding protein (ORF1p) of essential but uncertain function, and a 150 kDa ORF2 protein with endonuclease and reverse transcriptase (RT) activities ( … trimana 2049 century park eastNettetAn active LINE-1 encodes for 2 proteins translated from a single RNA containing two non-overlapping ORFs, ORF1 and ORF2. ORF2p is described in this entry as a … trimana 10920 wilshire blvdNettet19. apr. 2016 · Western blot analysis using antibodies specific to the human L1 ORF2 protein [42, 43] detected ORF2p in the total lysates of transfected HeLa cells (Fig. 5). … trimana woodland hills ca